IDENTIFICATION OF PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST -APPLICATIONS OF THE YEAST 2-HYBRID SYSTEM

Authors
GIETZ RD TRIGGSRAINE B ROBBINS A GRAHAM KC WOODS RA
Citation
Rd. Gietz et al., IDENTIFICATION OF PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST -APPLICATIONS OF THE YEAST 2-HYBRID SYSTEM, Molecular and cellular biochemistry, 172(1-2), 1997, pp. 67-79
Citations number
21
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
172
Issue
1-2
Year of publication
1997
Pages
67 - 79
Database
ISI
SICI code
0300-8177(1997)172:1-2<67:IOPTIW>2.0.ZU;2-K
Abstract
The yeast two-hybrid system is a molecular genetic test for protein in teraction. Here we describe a step by step procedure to screen for pro teins that interact with a protein of interest using the two-hybrid sy stem. This process includes, construction and testing of the bait plas mid, screening a plasmid library for interacting fusion proteins, elim ination of false positives and deletion analysis of true positives. Th is procedure is designed to allow investigators to identify proteins a nd their encoding cDNAs that have a biologically significant interacti on with your protein of interest.