ANALYSIS OF PHOSPHOLIPID MOLECULAR-SPECIES

Authors
VECCHINI A PANAGIA V BINAGLIA L
Citation
A. Vecchini et al., ANALYSIS OF PHOSPHOLIPID MOLECULAR-SPECIES, Molecular and cellular biochemistry, 172(1-2), 1997, pp. 129-136
Citations number
18
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
172
Issue
1-2
Year of publication
1997
Pages
129 - 136
Database
ISI
SICI code
0300-8177(1997)172:1-2<129:AOPM>2.0.ZU;2-P
Abstract
A method is described for analysing molecular species of glycerophosph olipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl-aikylacyl- and alke nylacyl-subclasses by HPLC on silicic acid. The molecular species of d iacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column.