EFFECT OF POLLINATION ON ACCUMULATION OF ACC SYNTHASE AND ACC OXIDASETRANSCRIPTS, ETHYLENE PRODUCTION AND FLOWER PETAL ABSCISSION IN GERANIUM (PELARGONIUM-X-HORTORUM BAILEY,L.H.)
Dg. Clark et al., EFFECT OF POLLINATION ON ACCUMULATION OF ACC SYNTHASE AND ACC OXIDASETRANSCRIPTS, ETHYLENE PRODUCTION AND FLOWER PETAL ABSCISSION IN GERANIUM (PELARGONIUM-X-HORTORUM BAILEY,L.H.), Plant molecular biology, 34(6), 1997, pp. 855-865
Self-pollination of diploid zonal geranium (Pelargonium x hortorum L.H
. Bailey) florets leads to a dramatic rise in ethylene production. fol
lowed by abscission within 4 h. Neither wounding of the stigma, pollin
ation with tetraploid pollen, nor heat-killed self pollen could elicit
as much ethylene production and petal abscission as self-pollination.
A cDNA sharing sequence identity with ACC synthase (GACS2) and three
different cDNAs sharing sequence identity with ACC oxidase (GACO1, GAC
O2, GACO3) were isolated from geranium pistils. Transcripts hybridizin
g with these probes increased slightly in response to self-pollination
, but the degree of accumulation in response to various treatments did
not correlate with ethylene production. When calculated on a per-plan
t-part basis, transcripts hybridizing with GACS2 were equally distribu
ted among the stigma+style, sterile ovary, and ovary tissues, but tran
scripts hybridizing with the three ACC oxidase clones were differentia
lly distributed. All transcripts were differentially expressed among t
he other tissues of the plant, with GACO1 being the most widely distri
buted. Ethylene production in geranium pistils was not autocatalytic.
Propylene failed to induce ethylene production and ethylene did not in
duce the accumulation of ACC synthase or ACC oxidase transcripts. ACC
accumulated in the stigma and style, and to a smaller extent in the st
erile ovary, after pollination. These data support a model of pollinat
ion-induced ethylene production by post-transcriptional regulation of
ethylene biosynthetic gene expression.