EXPRESSION OF AN ORTHOLOG OF REPLICATION PROTEIN A1 (RPA1) IS INDUCEDBY GIBBERELLIN IN DEEP-WATER RICE

Citation
E. Vanderknaap et al., EXPRESSION OF AN ORTHOLOG OF REPLICATION PROTEIN A1 (RPA1) IS INDUCEDBY GIBBERELLIN IN DEEP-WATER RICE, Proceedings of the National Academy of Sciences of the United Statesof America, 94(18), 1997, pp. 9979-9983
Citations number
46
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
94
Issue
18
Year of publication
1997
Pages
9979 - 9983
Database
ISI
SICI code
0027-8424(1997)94:18<9979:EOAOOR>2.0.ZU;2-U
Abstract
Internodes of deepwater rice are induced to grow rapidly when plants b ecome submerged. This adaptation enables deepwater rice to keep part o f its foliage above the rising flood waters during the monsoon season and to avoid drowning. This growth response is, ultimately, elicited b y the plant hormone gibberellin (GA),The primary target tissue for GA action is the intercalary meristem of the internode. Using differentia l display of mRNA, we have isolated a number of genes whose expression in the intercalary meristem is regulated by GA. The product of one of these genes was identified as an ortholog of replication protein A1 ( RPA1). RPA is a heterotrimeric protein involved in DNA replication, re combination, and repair and also in regulation of transcription. A chi meric construct, in which the single-stranded DNA-binding domain of ri ce RPA1 was spliced into the corresponding region of yeast RPA1, was a ble to complement a yeast rpa1 mutant. The transcript level of rice RP A1 is high in tissues containing dividing cells. RPA1 mRNA levels incr ease rapidly in the intercalary meristem during submergence and treatm ent with GA before the increase in the level of histone H3 mRNA, a mar ker for DNA replication.