EFFECTS OF FIBRONECTIN FRAGMENTS ON DNA TRANSFECTION INTO MAMMALIAN-CELLS BY ELECTROPORATION

Two kinds of cells, human epidermoid carcinoma A431 cells and simian k idney COS-7 cells, were transfected with the chloramphenicol acetyl tr ansferase (CAT) gene by electroporation, and then cultivated on cultur e dishes coated with two different forms of recombinant fibronectin fr agments consisting of cell-binding domain (C-274) or heparin-binding d omain and CS1 region (H-296), In the case of A431 cells, H-296-coated dishes significantly increased the amount of expressed CAT and the adh esion of electroporated cells in comparison with non-coated dishes. C- 274 was effective for COS-7 cells. Overall, these fibronectin fragment s increased the recovery of the transfectants on A-431 cells and COS-7 cells, respectively.