GENOME ANALYSIS ON LINKAGE GROUP-VI OF NEUROSPORA-CRASSA

Two chromosome walks covering 420 and 110 kb on the left arm of linkag e group VI (LGVIL) of Neurospora crassa were pursued with the goal of cloning carotenogenic loci. Complementation analysis with clones isola ted in the 420-kb walk allowed identification of the yellow-1 (ylo-1) gene which is essential for Neurospora carotenogenesis. We have physic ally located a second gene, unknown-13(un-13), between the cross-pathw ay control-1, (cpc-1) and ylo-1 loci. Cloning of a second potential ca rotenogenic locus, vivid(vvd), from our walks was attempted using scre ening of Northern blots with radiolabeled DNA fragments from walk clon es to identify gene transcripts. The radiolabeled DNA fragments were u sed to clone complementary DNA isolates representing an additional fou r genes in the two walks. (C) 1997 Academic Press.