SUGAR ACQUISITION DURING THE DEVELOPMENT OF MICROSPORIDIAN (MICROSPORA, NOSEMATIDAE) SPORES

Citation
Ah. Undeen et Lf. Solter, SUGAR ACQUISITION DURING THE DEVELOPMENT OF MICROSPORIDIAN (MICROSPORA, NOSEMATIDAE) SPORES, Journal of invertebrate pathology, 70(2), 1997, pp. 106-112
Citations number
19
Categorie Soggetti
Zoology
ISSN journal
00222011
Volume
70
Issue
2
Year of publication
1997
Pages
106 - 112
Database
ISI
SICI code
0022-2011(1997)70:2<106:SADTDO>2.0.ZU;2-C
Abstract
Helicoverpa tea larvae were infected with Vairimorpha necatrix. The fa t body was triturated and sporulation stages were fractionated accordi ng to buoyant density by Ludox density gradient centrifugation, Spores and sporulation stages formed two minor bands with buoyant densities of 1.072 and 1.121 g/ml, and two major bands with buoyant densities of 1.150 and 1.198 g/ml. The higher bands of less dense sporoblastic sta ges first appeared 96 hr following infection and the band containing t he heaviest (1.198 g/ml) and most refringent mature spores appeared la st, ca. 24 hr after the appearance of the other bands, Although the ba nds remained in the same relative positions regardless of the time aft er inoculation, the percentage of spores in Band 4 increased with time . The concentration of sugar in the spores from the 1.198-g/ml band wa s more than four times the concentration found in spores in the 1.150- g/ml band and accounted for more than 60% of the increase in spore wei ght between the two density classes. Similar tests with Nosema algerae yielded similar results, with sugars accounting for 88% of the increa sed spore weight. Sugar acquisition appears to occur during the attain ment of the final spore density and perhaps signals spore maturation. (C) 1997 Academic Press.