Wy. Su et T. Gordon, IN-VIVO EXPOSURE TO OZONE PRODUCES AN INCREASE IN A 72-KDA HEAT-SHOCK-PROTEIN IN GUINEA-PIGS, Journal of applied physiology, 83(3), 1997, pp. 707-711
Although several lines of evidence have suggested that oxidizing agent
s can induce heat shock proteins (HSPs) in vitro, little is known abou
t the induction of HSPs during in vivo exposure to oxidants. Guinea pi
gs were exposed to ozone for 6 h and euthanized up to 72 h later. Prot
eins from lavage cells and lung tissue were characterized by immunoblo
tting with 72- and 73/72-kDa HSP monoclonal antibodies. Although 73-kD
a HSP was expressed constituitively in lung tissue, it was not affecte
d by ozone. In contrast, 72-kDa HSP was significantly increased in lav
age cells and lung tissue of animals exposed to 0.4 and 0.66 parts/mil
lion of ozone. Both heat treatment and arsenite induced 72-kDa HSP in
cultured alveolar macrophages. The increase in 72-kDa HSP in the lavag
e cell pellet peaked at 24 h after ozone, whereas the influx of polymo
rphonuclear leukocytes peaked at 4 h. Examination of the induction of
HSPs by ozone may provide clues to the development of ozone tolerance
in humans and animals.