SEPARATION AND CHARACTERIZATION OF THE TRYPTIC PEPTIDE-MAPPING OF RECOMBINANT BOVINE GROWTH-HORMONE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY

Reversed phase high-performance liquid chromatography (RPHPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was used to s eparate and characterize the peptides resulting from the tryptic cleav age of somidobove, a recombinant bovine growth hormone, The tryptic di gestion of somidobove was carried out at room temperature for 15 hours , The tryptic peptides sere separated on a Zorbax SB300 C8 column with trifluoroacetic acid - acetonitrile gradient elution and characterize d by LC/ESI-MS. Resulting single and combined peptide fragments were c haracterized by comparing the observed molecular mass with the calcula ted mass for these fragments. (C) 1997 by John Wiley & Sons, Ltd.