A SOLID-PHASE ASSAY TO SCREEN MONOCLONAL-ANTIBODIES AGAINST DNA-BINDING PROTEIN

Authors
ISHII N NAKAYAMA H KATAYAMA J ARISAWA M AOKI Y
Citation
N. Ishii et al., A SOLID-PHASE ASSAY TO SCREEN MONOCLONAL-ANTIBODIES AGAINST DNA-BINDING PROTEIN, Nucleic acids research, 25(17), 1997, pp. 3550-3551
Citations number
8
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
25
Issue
17
Year of publication
1997
Pages
3550 - 3551
Database
ISI
SICI code
0305-1048(1997)25:17<3550:ASATSM>2.0.ZU;2-I
Abstract
A method is described for selecting monoclonal antibodies (mAb) agains t DNA-binding protein, The protocol involves a non-radioactive solid-p hase DNA binding assay using a 96-well plate. Because the solid-phase assay is highly specific and sensitive, partially purified antigen is sufficient for the immunization, and mAb screening can be performed wi th crude cell extract as the antigen, MAbs obtained by this method cou ld supershift the DNA-protein complex in the electromobility shift ass ay, and were sufficient for immunoscreening of a cDNA expression libra ry.