PROMINENT ROLE OF INTRACELLULAR CA2-ARTERY( RELEASE IN HYPOXIC VASOCONSTRICTION OF CANINE PULMONARY)

1 The possible role of sarcoplasmic reticulum (SR) Ca2+ stores in hypo xic pulmonary vasoconstriction (HPV) is not well understood. In order to assess the possible role of intracellular Ca2+ release from SR Ca2 stores in HPV, we examined the effects of: (1) ryanodine (10 mu M) de pletion of intracellular Ca2+ stores, and (2) thapsigargin (THAPS, 2 m u M) or cyclopiazonic acid (CPA, 10 mu M) depletion of intracellular C a2+ stores on HPV in canine pulmonary artery. 2 Isometric tension was measured from arterial ring suspended in Krebs-Henseliet solution (K-H ) bubbled with 95%O-2/5%CO2. Hypoxia was induced by bubbling phenyleph rine (PE, 1 mu M) precontracted rings with 95%N-2/5%CO2. HPV was obser ved in bath intact and endothelial-denuded arteries and expressed as % of maximal KCI contraction (%T-kmax)=21.3 +/- 3.2%; n=13 and 21.7 +/- 4%; n=4, respectively. 3 When SR caffeine sensitive Ca2+ stores were depleted by pretreatment with ryanodine and brief caffeine (15 mM) exp osure, the hypoxic response was significantly reduced to 19.1 +/- 9.2% of the control hypoxic contraction (n=7; P<0.001) with little or no e ffect on PE or KCl contractions. On the other hand, in normoxic rings pretreated with THAPS or CPA, the PE responses were significantly redu ced (%T-kmax=18.2 +/- 3.1% compared to 39.0 +/- 3.9% in control; n=16; P<0.001; %T-kmax=3.4 +/- 1.6% compared to 49.9 +/- 7.9% in control, n =6; P<0.001; respectively) with no significant effect on caffeine-indu ced contractions, suggesting that both THAPS and CPA preferentially de plete InsP(3)-sensitive Ca2+ stores, without affecting the caffeine-se nsitive Ca2+ store; consistent with the existence of separate and inde pendent InsP(3) and caffeine-sensitive Ca2+ stores in this preparation . 4 When hypoxia was induced in the presence of THAPS or CPA, develope d tension was significantly larger than control (%T-kmax=64.5 +/- 6.0% ; n=16; P<0.05%; %T-kmax=78.2 +/- 15%; n=6; P<0.05; respectively), was partially blocked by nisoldipine (10 mu M) and ryanodine (%T-kmax=20. 3 +/- 3.7%; n=6), and nearly completely blocked by SK&F 96365 (50 mu M ). However, the actions of SK&F 96365 appeared to be nonselective sinc e this compound also significantly reduced contractions elicited by KC l, PE and caffeine. 5 Finally, evidence was obtained suggesting: (a) t hat at least some of the Ca2+ released from the caffeine-and ryanodine -sensitive Ca2+ stores by hypoxia may be taken up and buffered by the InsP(3)-sensitive Ca2+ stores, and (b) the apparent dependence of HPV on extracellular Ca2+ entry pathways may be partially due to the depen dence of the Ca2+ content of intracellular SR Ca2+ stores on sarcolemm al Ca2+ entry pathways. 6 These data suggest that caffeine-and ryanodi ne-sensitive SR Ca2+ stores contribute significantly to HPV under norm al conditions and, in the presence of THAPS or CPA, an additional niso ldipine-and ryanodine-insensitive Ca2+ entry pathway is evoked by hypo xia.