DETERMINATION OF FREE AMINO-ACIDS IN PIG PLASMA BY PRECOLUMN DERIVATIZATION WITH 6-N-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic method for measuring amino a cids in pig plasma has been developed by using 6-aminoquinolyl-N-hydro xysuccinimidyl carbamate as a precolumn derivatization reagent. With t he method presented here it was possible to separate 24 amino acids in pig plasma within one single run in 45 min, while only 18 amino acids were properly separated with the original AccQ.Tag conditions used fo r analysis of hydrolysate samples. The recovery was above 90% for most amino acids, and the mean coefficient of variation of the retention t imes below 0.30%. The within-and between-assay reproducibility for the determination of plasma amino acids showed C.V.s below 2.2% and 4.5%, respectively, for most amino acids analyzed. In the present work, mos t of the plasma amino acids were assayed with high sensitivity, accura cy and good reproducibility in a relatively short time and on very sma ll amounts of sample. (C) 1997 Elsevier Science B.V.