SERUM-PROTEIN DETERMINATION BY HIGH-PERFORMANCE GEL-PERMEATION CHROMATOGRAPHY

A general high-performance gel-permeation chromatography (HPGPC) metho d was developed to determine protein in human serum with improved sens itivity and speed. The optimum UV wavelength for protein detection was found to be 210 nm, by comparing the protein values obtained by varyi ng the UV wavelength of the HPLC detection system with the protein val ues obtained from spectrophotometric protein assays, i.e., the bicinch oninic acid (BCA) method and the biuret method. The analysis time was less than 1 min. Since this HPGPC serum protein assay method is simple and rapid, it is expected to be particularly well adapted for use in clinical laboratories. (C) 1997 Elsevier Science B.V.