The principle of luminescent systems (firefly and bacterial biolumines
cence, luminol, dioxetane and acridans chemiluminescence) is described
. The bio-and chemiluminescent reagents can be employed as highly sens
itive and, together with immobilized enzymes, also specific detection
systems in bioanalytical assays both by using batch determination and
continuous flow systems. An example of versatile manifold (flow sensin
g device) is shown. A list of possible bioanalytical applications is g
iven.