THE UTILIZATION OF CAPILLARY ZONE ELECTRO PHORESIS FOR THE ANALYTICALEVALUATION OF TINCTURA-OPII

A simple and rapid method suitable for the analytical evaluation of '' Tinctura opii'' preparation has been proposed. Optimization of the con ditions for capillary zone electrophoresis (CZE) was based mainly on t he evaluation of the influence of beta-cyclodextrin (beta-CD) and its synthetic derivatives dimethyl-beta-cyclodextrin and carboxymethyl-bet a-cyclodextrin (CM-beta-CD) on the separation of morphine, codeine, th ebaine, and papaverine as major alkaloid constituents of ''Tinctura op ii''. The best separation was achieved using the background electrolyt e consisting of 30 mM aspartic acid 30 mM beta-alanine (pH 3.7) with t he addition of 5 mM CM-beta-CD. Experimental results demonstrate a hig h separation power of CD-modified CZE for the structure related morphi ne alkaloids.