INACTIVATION OF POLIOVIRUS WITH CHLORAMIN ES

Inactivation rates for poliovirus (Lsc, 2ab strain), suspended in a ph osphate buffer salt solution (PBS) and a cell maintenance medium (MEM) , with chloramines, N-chlorosuccinimide (Cl-SI), chloramine T (Cl-T), ammoniamonochloramine (NH2Cl), N-chlorodiethylamine (Cl-DEA) and N-chl oroglycine (Cl-Gly) at 2 and 10 mg/l as available chlorine (Cl-2),were determined at pH 7 and 20 degrees C. Inactivation efficacies of these chloramines were compared each other and discussed in relation to che mical potency, hydrolysis constants and chlorination rates for the chl oramines. At 10 mg/l as Cl-2, the periods for the 99% inactivation of the virus suspended in PBS with Cl-SI. Cl-T, and NH2Cl were 2.5, 5, an d 5 min, respectively, However, Cl-DEA and Cl-Gly did not reach 99% in activation in 10 min contact. There were a little chlorine consumption in the test solutions of Cl-SI, Cl-T, and NH2Cl but of Cl-DEA and Cl- Gly. The inactivation efficacies of these chloramines did not correspo nd to the order of their hydrolysis constants. The faster chlorination rate for Cl-SI than tested other chloramines may have an influence on its faster inactivation rate, Inactivation rates for the virus suspen ded in MEM with the chloramines were slower than that for the virus su spended in PBS. It might be due to the formation of less effective chl oramines from constituents in MEM virus suspension.