A 2-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY PROCEDURE FOR SIMULTANEOUS SEPARATION OF CERAMIDE AND DIACYLGLYCEROL SPECIES

We have developed a novel, simple, and rapid, two-dimensional thin-lay er chromatography method to separate 1,2-, 1,3-diacylglycerols and cer amides containing alpha-hydroxy and normal fatty acids from other neut ral lipids on one 10 x 10 cm precoated silica gel plate. The three sol vent systems used in succession leave the phospholipids at the origin and separate neutral lipids of interest into component species. We hav e applied this method to incorporation of 9,10[H-3]myristic acid into lipids of gills from sea bass and obtained results that are similar an d comparable to those obtained by described methods.