STUDY ON GENE SENSOR-BASED ON PRIMER EXTENSION

Based on the fact that the resonant frequency of a piezoelectric cryst al is the function of its surface deposit, and that the primer extends after it hybridizes with the template, the primer extension gene sens or technique was developed. The prominent feature of the technique is that fast and sensitive frequency signals are used as the monitoring s ystem of gene hybridization and primer strand extension. Results show that this technique may be used in homologous analysis of nucleic acid , trace DNA detection, and determining the integration of DNA. It may also be used for isolation of target gene, gene mutation analysis, and predicting the location of a gene in its genome.