Yq. Xiao et al., POSSIBLE PARTICIPATION OF MACROPHAGE INFLAMMATORY PROTEIN-2 IN NEUTROPHIL INFILTRATION IN ALLERGIC INFLAMMATION IN RATS, Biochimica et biophysica acta. Molecular basis of disease, 1361(2), 1997, pp. 138-146
Recombinant rat macrophage inflammatory protein 2 (MIP-2) was prepared
from E, coli transfected with a glutathione-S-transferase (GST)-MIP-2
fusion protein expression vector. A polyclonal antibody to rat MIP-2
was then obtained from rabbits by immunization with recombinant rat MI
P-2. Using the polyclonal antibody which selectively suppressed neutro
phil chemotactic activity of MIP-2, the role of MIP-2 in neutrophil in
filtration in allergic inflammation in rats was studied. In an air pou
ch-type allergic inflammation model in rats, neutrophil infiltration i
nto the pouch fluid increased with time after antigen challenge. Neutr
ophil chemotactic activity in the pouch fluid collected 8 h after anti
gen challenge was diminished by anti-MIP-2 antibody. In addition, when
leukocytes that had infiltrated into the pouch fluid collected 4 h af
ter antigen challenge were incubated, neutrophil chemotactic activity
in the conditioned medium increased time-dependently, and the activity
was neutralized by anti-MIP-2 antibody. Furthermore, when anti-MIP-2
antibody was injected into the pouch 6 h after antigen challenge, neut
rophil infiltration into the pouch fluid during the next 2 h was suppr
essed. These findings indicate that MIP-2 plays an important role in n
eutrophil infiltration in rat allergic inflammation. (C) 1997 Elsevier
Science B.V.