DETERMINATION OF MYCOTOXINS PRODUCED BY FUSARIUM ISOLATES FROM BANANAFRUITS BY CAPILLARY GAS-CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method of analysis for trichothecenes (nivalenol, deoxynivalenol, 3- and 15-acetyldeoxynivalenol, diacetoxyscirpenol, neosolaniol, T-2 tet raol, T-2 and HT-2 toxins), zearalenone and zearalenols, and another m ethod for determination of fumonisin B-1 are described and applied to cultures of Fusarium isolated from bananas. Both methods were adapted from different techniques of extraction, clean-up and determination of these mycotoxins. The first method involves extraction with methanol- 1% aqueous sodium chloride, clean-up of extracts by partition with hex ane and dichloromethane, additional solid reversed-phase clean-up and analysis of two eluates by both high-performance liquid chromatography with ultraviolet detection and capillary gas chromatography. The meth od for fumonisin B-1 implies extraction with aqueous methanol, concent ration, clean-up with water and methanol on Amberlite XAD-2 column, fo rmation of a fluorescent 4-fluoro-7-nitrobenzofurazan derivative and a nalysis by high-performance liquid chromatography with fluorescence de tection. Both procedures give good limits of detection and recoveries, and are considered suitable for the detection and quantification of t he studied toxins in corn and rice cultures of Fusarium spp. isolated from banana fruits. (C) 1997 Elsevier Science B.V.