SIMULTANEOUS DETERMINATION OF VITAMINS-A AND VITAMIN-E IN RAT-TISSUESBY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic (HPLC) method to determine v itamins A, Ap and E simultaneously was developed with direct extractio n of vitamins from rat tissues with n-hexane and probe sonicating. The dry residue was redissolved in chloroform-methanol. Vitamins A and Ap were detected by UV-Vis and vitamin E by fluorescence. Vitamin K, use d as internal standard, was detected both by UV-Vis and by fluorescenc e. Standards and samples were checked for linearity giving correlation coefficients that were higher than 0.99 in the concentration range of 3.1-9.4 for vitamin A, 8.2-24.7 for vitamin Ap and 0.6-1.7 nmol/g in the case of liver extracts and 0.5-3.0 nmol/g in the case of placenta. The intra-assay precision (R.S.D.) varied between 1.48 and 7.25, wher eas inter-assay precision was between 4.99 and 7.03. Recoveries ranged between 94+/-8 and 107+/-5%. Results from the application of this met hod to different rat tissues having wide range of vitamin content are presented. (C) 1997 Elsevier Science B.V.