MONOCLONAL-ANTIBODY E8-18 IDENTIFIES AN INTEGRAL MEMBRANE-SURFACE PROTEIN UNIQUE TO MYCOPLASMA-CAPRICOLUM SUBSP CAPRIPNEUMONIAE

Authors
RURANGIRWA FR SHOMPOLE PS WAMBUGU AN KIHARA SM MCGUIRE TC
Citation
Fr. Rurangirwa et al., MONOCLONAL-ANTIBODY E8-18 IDENTIFIES AN INTEGRAL MEMBRANE-SURFACE PROTEIN UNIQUE TO MYCOPLASMA-CAPRICOLUM SUBSP CAPRIPNEUMONIAE, Clinical and diagnostic laboratory immunology, 4(5), 1997, pp. 615-619
Citations number
49
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
Journal title
Clinical and diagnostic laboratory immunology → ACNP
ISSN journal
1071412X
Volume
4
Issue
5
Year of publication
1997
Pages
615 - 619
Database
ISI
SICI code
1071-412X(1997)4:5<615:MEIAIM>2.0.ZU;2-2
Abstract
Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplas ma capricolum subsp. capripneumoniae in Western blots identifying an e pitope on a 24 kDa antigen (p24). MAb E8-18 did not react with II isol ates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of tryp sin treatment of intact organisms and detergent-phase partitioning rev ealed p24 to be an integral M. capricolum subsp. capripneumoniae surfa ce membrane protein.