THE EFFECT OF POLYAMINES ON THE DEVELOPMENT OF SUGAR-BEET PROTOPLASTS

The influence of the exogenous polyamines: putrescine, spermidine and spermine, on the frequency of protoplast divisions for 2 genotypes of sugar beet (Beta vulgaris L.) was analyzed. Protoplasts were cultured by the agarose disk method on Saunders and Doley medium supplemented w ith either hormones or polyamines, or hormones combined with polyamine s. The latter supplement led to a statistically significant increase i n prating efficiency. The improvement in division index was caused mai nly by spermine.