DIAGNOSIS OF DENGUE BY USING REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION

Authors
MIAGOSTOVICH MP DOSSANTOS FB DEARAUJO ESM DIAS J SCHATZMAYR HG NOGUEIRA RMR
Citation
Mp. Miagostovich et al., DIAGNOSIS OF DENGUE BY USING REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION, Memorias do Instituto Oswaldo Cruz, 92(5), 1997, pp. 595-599
Citations number
26
Categorie Soggetti
Tropical Medicine
Journal title
Memorias do Instituto Oswaldo Cruz → ACNP
ISSN journal
00740276
Volume
92
Issue
5
Year of publication
1997
Pages
595 - 599
Database
ISI
SICI code
0074-0276(1997)92:5<595:DODBUR>2.0.ZU;2-G
Abstract
A rapid identification of dengue viruses from clinical samples by usin g a nested reverse transcriptase-polymerase chain reaction (RT-PCR) pr ocedure was carried out for diagnostic and epidemiological purposes. R T-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue vi ruses in suspected cases, allowing a rapid identification of new serot ypes in endemic areas.