COMPLEXES OF FE2-METHYL-D-GLUCAMINE DITHIOCARBAMATE AS TRAPS OF NITRIC-OXIDE IN ANIMAL-TISSUES - COMPARATIVE INVESTIGATIONS( WITH DIETHYLDITHIOCARBAMATE OR N)

In EPR experiments on mice it was demonstrated that a hydrophobic comp lex Fe2+ with diethyldithiocarbamate (DETC) is a more efficient select ive NO trap than a hydrophilic complex Fe2+ with N-methyl-D-glutamine dithiocarbamate (MGD). This difference can be due to the higher stabil ity of paramagnetic nitrosyl iron complex with DETC (MNIC-DETC) formed by NO binding to Fe2+-DETC in animal tissues in vivo. The complex ana logue MNIC-MGD is reversibly oxidized in animal blood to transform int o the diamagnetic EPR-silent form. The latter is detectable also in ur ine of animals, especially of those treated with bacterial lipopolysac charide which initiates the enhanced NO production in the organism. We suggest that NO2 or peroxynitrite formed from endogenous NO can serve as an agent reversibly oxidizing MNIC-MGD in these animals.