ACCUMULATION OF STRESS-PROTEINS IN INTERCELLULAR SPACES OF BARLEY LEAVES INDUCED BY BIOTIC AND ABIOTIC FACTORS

Accumulation of the extracellular proteins localized in intercellular spaces of barley primary leaves was examined after inoculation with po wdery mildew (Erysiphe graminis f. sp. hordei) as biotic stress factor and after abiotic stresses such as heat shock, low temperature and he avy metal (Mg, Zn, Cu, Al, Cd and Co) treatment using native polyacryl amide gel electrophoresis. Six to eight major pathogen-induced protein s (bands on native gel) have been identified. Their accumulation at ho st-parasite incompatibility was more expressive than at compatibility interaction. Elevated temperature did not induce pathogenesis-related (PR) proteins while low temperature induced three of them. Cu, Al, Cd and Co induced accumulation pattern of extracellular proteins was very similar to that in powdery mildew inoculated leaves. Mg and Zn had no effect on the induction of protein accumulation in the intercellular spaces of leaves. Induction of PR proteins by different stresses indic ated their general function in the resistance of plants to changing en vironment.