DETECTION OF BK VIRUS IN URINE BY POLYMERASE CHAIN-REACTION - A COMPARISON OF DNA EXTRACTION METHODS

Authors
BEHZADBEHBAHANI A KLAPPER PE VALLELY PJ CLEATOR GM
Citation
A. Behzadbehbahani et al., DETECTION OF BK VIRUS IN URINE BY POLYMERASE CHAIN-REACTION - A COMPARISON OF DNA EXTRACTION METHODS, Journal of virological methods, 67(2), 1997, pp. 161-166
Citations number
27
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
67
Issue
2
Year of publication
1997
Pages
161 - 166
Database
ISI
SICI code
0166-0934(1997)67:2<161:DOBVIU>2.0.ZU;2-L
Abstract
Using specimens spiked with BK virus, several DNA extraction methods w ere evaluated for their ability to remove polymerase chain reaction (P CR) inhibitors from urine samples. It was found that PCR inhibition co uld be completely overcome by extracting samples with 30% polyethylene glycol (PEG) in 3 M sodium chloride, and partially overcome by extrac ting samples with guanidine thiocyanate in the presence of high salt c oncentrations. The nature of the sample inhibition was investigated, l eading to the conclusion that both urea and unidentified non-proteinac eous DNA associated substances inhibit BKV DNA amplification from urin e. (C) 1997 Elsevier Science B.V.