THERAPEUTIC DRUG-MONITORING OF RISPERIDONE AND 9-HYDROXYRISPERIDONE IN SERUM WITH SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

This laboratory developed a simple and efficient solid-phase extractio n method that is combined with high-performance liquid chromatography for rapid and precise therapeutic monitoring of risperidone (Risperdal ) in blood concentrations. The solid-phase extraction uses a mixed bed column. Sensitivity of the chromatographic method is 0.5 ng/ml (180 p mol/ml) of drug in serum, and separations can be performed in a 15-min ute chromatographic run. Advantages of this approach include enhanced speed, sensitivity, and efficiency. A high level of sensitivity may be achieved because of the absence of interference from other drugs, met abolites, or serum components.