DIRECT MONITORING OF ORGANIC-REACTIONS ON POLYMERIC SUPPORTS

A method to use matrix-assisted laser desorption/ionization mass spect rometry (MALDI-MS) for real-time monitoring of organic reactions on po lymeric supports used in solid-phase synthesis is described. The strat egy utilizes a synthetic construct that allows for the rapid and conve nient direct MALDI analysis of the attached substrates as well as thei r subsequent chemical cleavage as desired. We have used this strategy to monitor nucleophilic substitutions, palladium-catalyzed coupling re actions, and solid phase peptide synthesis reactions using both Boc- a nd Fmoc-based chemistries. (C) 1997 Elsevier Science Ltd.