COSMID CONTIG AND CDNA MAP OF THE HUMAN-CHROMOSOME 13Q14 REGION FREQUENTLY LOST IN B-CELL CHRONIC LYMPHOCYTIC-LEUKEMIA

We constructed a fine physical map of human chromosome 13q14 region be tween D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. T his interval had been shown to be in the center of the genome region f requently lost in a human blood malignancy known as B-cell chronic lym phocytic leukemia (BCLL), Mapping of the genome region is a step in se arching for a putative tumor suppressor gene for BCLL. A chr13-specifi c cosmid library (LA13NC01) was screened with four YAC and seven WI-ST S markers belonging to the above 13q14 interval, yielding a cosmid sub set of more than 400 clones representing the region. Cosmids between D 13S1168 and D13S25 loci were arranged in contigs. Seven different clon es were found in cDNA libraries by hybridization screening with YAC IC RF66c1 and two cosmids covering the D13S319 locus, which is the centra l point for BCLL-associated deletions, The cDNA clones were mapped aga inst the contigous cosmids.