AN APPROACH TO IDENTIFY FUNCTIONAL HOMOLOGS AND SUPPRESSORS OF GENES IN FISSION YEAST

We have developed a procedure using a bank of temperature-sensitive (t s) mutants of fission yeast to identify mutants which can be rescued b y expression of a plasmid-borne gene of interest. The procedure has be en used to identify new ts alleles of cdc2 and swi7/pol1, a ts mutant rescued by actin, and to identify a ts allele of cdc11 which can be re scued by combined mammalian Myc and Max expression. The procedure shou ld also be useful as an alternative approach to identify genes in fiss ion yeast which are functionally homologous to genes of interest from other organisms.