FINGERPRINTING AND PEDIGREE ANALYSIS IN EUCALYPTUS-GLOBULUS USING RAPDS

RAPDs were used to confirm clonal fidelity and distinguish individuals of varying degrees of relationship in Eucalyptus globulus. RAPD varia tion was examined amongst ramets derived from 10 ortets which were eit her Full-sibs, half-sibs, or unrelated, as well as heir parents. Genet ic similarity amongst samples was calculated from 117 RAPD bands using the simple matching coefficient. RAPD variation within clones was tri vial compared to the variation found even between full-sib clones. Gen etic similarity decreased with pedigree distance. The pedigree of the material was successfully displayed by UPGMA clustering of the genetic similarity matrix, with parents generally clustering with their offsp ring. In a second experiment, 120 RAPD bands allowed the successful di scrimination of selfs from their intra- and interprovenance half-sib o utcrosses and again genetic similarity decreased with pedigree distanc e. These results clearly indicate that RAPDs have great potential for estimation of outcrossing rates and pedigree analysis.