STH132I, A NOVEL CLASS-IIS RESTRICTION-ENDONUCLEASE OF STREPTOCOCCUS-THERMOPHILUS ST132

The Sth132I restriction endonuclease (R.Sth132I) was detected in Strep tococcus thermophilus ST132 and purified to near homogeneity by hepari n Sepharose CL-6B affinity chromatography. Fragments from Sth132I dige stion of plasmid DNA were subcloned into pUC19 in Escherichia coli DH5 alpha and sequenced. Sequence analysis of inserts and their ligation junction sites revealed that Sth132I is a novel class-IIS restriction endonuclease, which recognizes the non-palindromic sequence 5'-CCCG(N) (4)-3' 3'-GGGC(N)(8)-5'. (C) 1997 Elsevier Science B.V.