E. Kaltchuksantos et al., CYTOLOGICAL ANALYSIS OF EARLY MICROSPORE DIVISIONS AND EMBRYO FORMATION IN CULTURED SOYBEAN ANTHERS, Plant cell, tissue and organ culture, 49(2), 1997, pp. 107-115
Young inflorescences of two Brazilian soybean cultivars (IAS 5 and RS
7) were subjected to 4 degrees C pretreatment for 0, 5, and 10 days. C
ytological examinations of the lit vitro anthers were done during the
first four weeks of culture. The cold pretreatment had no clear effect
on the frequencies of symmetrical-binucleate microspores or multinucl
eate grains. The multinucleate grains might originate either by symmet
rical or assymmetrical division. The best medium for callus and embryo
induction was B5 long containing 2.0 mg l(-1) 2,4-dichlorophenoxy ace
tic acid and 0.5 mg l(-1) benzyladenine. The frequency of anthers/call
i with embryos was about 2% in all cultivars. Histological analysis of
such embryos showed that they were similar to zygotic embryos.