CHINESE-HAMSTER PROTEIN HOMOLOGOUS TO HUMAN PUTATIVE PROTEIN-KINASE KIAA0204 IS ASSOCIATED WITH NUCLEI, MICROTUBULES AND CENTROSOMES IN CHO-K1 CELLS

Monoclonal antibody raised against a preparation of leach fish sperm c entrosomes was used for screening of cDNA expressing library of Chines e hamster CHO-K1 cells, Two positive clones appeared to encode 628 ami no acid protein fragment that was 72% identical to human KIAA0204 prot ein, i.e. putative protein kinase. Polyclonal antibodies raised agains t products of cDNA expression in E. coli recognized 210-kDa polypeptid e in CHO-K1 cells and immunostained nuclear speckles, centrosomes and microtubules in these cells, The 210-kDa polypeptide (named MAK-L) co- sedimented with exogenous microtubules. Thus, one more protein kinase seems to be associated with the microtubule network in vertebrate cell s. (C) 1997 Federation of European Biochemical Societies.