SELECTION OF DOWN-REGULATED SEQUENCES ALONG THE MONOCYTIC DIFFERENTIATION OF LEUKEMIC HL60 CELLS

In order to dissect the molecular mechanisms of monocytic differentiat ion we have developed a subtractive hybridisation method based on a si mplified 'representational difference analysis', We have selected 16 s equences and confirmed their down-regulation along the TPA-induced mon ocytic differentiation of HL60 cells, Among these sequences we have id entified the alpha-tubulin, the TaxREB protein and two ribosomal prote in sequences which had mat been previously described as differentially expressed, These results add to our knowledge about the molecules imp licated along the monocytic differentiation and growth arrest of leuke mic cells and provide a first step in the study of their respective ro les. (C) 1997 Federation of European Biochemical Societies.