TYPING TUBER ECTOMYCORRHIZAE BY POLYMERASE CHAIN AMPLIFICATION OF THEINTERNAL TRANSCRIBED SPACER OF RDNA AND THE SEQUENCE CHARACTERIZED AMPLIFIED REGION MARKERS

Identification of some economically important Tuber species using clas sical morphological characteristics is sometimes difficult. We report here the molecular characterization of a species coming from China, Tu ber indicum, mistaken with Tuber melanosporum species. Using restricti on analysis of the amplified internal transcribed spacer (ITS) of rDNA , ITS sequence analysis, and sequence characterized amplified region m arkers, with DNA from fruit bodies or mycorrhizae, genetic variation w as found between these two species, allowing to differentiate and char acterize them.