E. Wehmann et al., RAPID IDENTIFICATION OF NEWCASTLE-DISEASE VIRUS-VACCINE STRAINS LASOTA AND B-1 BY RESTRICTION SITE ANALYSIS OF THEIR MATRIX GENE, Vaccine, 15(12-13), 1997, pp. 1430-1433
A region constituting 88% of the matrix gene of Newcastle disease viru
s vaccine strains LaSota and B-1 was amplified by reverse transcriptio
n-polymerase chain reaction. Amplified products of LaSota and B-1 stra
ins derived from vaccine serials of different companies were digested
with restriction enzymes MboI and HinfI. Strain characteristic cleavag
e site maps were obtained that allowed for a reliable and rapid differ
entiation between strains LaSota and B-1. (C) 1997 Elsevier Science Lt
d.