RAPID IDENTIFICATION OF NEWCASTLE-DISEASE VIRUS-VACCINE STRAINS LASOTA AND B-1 BY RESTRICTION SITE ANALYSIS OF THEIR MATRIX GENE

Citation
E. Wehmann et al., RAPID IDENTIFICATION OF NEWCASTLE-DISEASE VIRUS-VACCINE STRAINS LASOTA AND B-1 BY RESTRICTION SITE ANALYSIS OF THEIR MATRIX GENE, Vaccine, 15(12-13), 1997, pp. 1430-1433
Citations number
21
Categorie Soggetti
Immunology
Journal title
ISSN journal
0264410X
Volume
15
Issue
12-13
Year of publication
1997
Pages
1430 - 1433
Database
ISI
SICI code
0264-410X(1997)15:12-13<1430:RIONVS>2.0.ZU;2-Z
Abstract
A region constituting 88% of the matrix gene of Newcastle disease viru s vaccine strains LaSota and B-1 was amplified by reverse transcriptio n-polymerase chain reaction. Amplified products of LaSota and B-1 stra ins derived from vaccine serials of different companies were digested with restriction enzymes MboI and HinfI. Strain characteristic cleavag e site maps were obtained that allowed for a reliable and rapid differ entiation between strains LaSota and B-1. (C) 1997 Elsevier Science Lt d.