A SIMPLIFIED METHOD FOR THE PREPARATION OF ALPHA-P-32-LABELED DEOXYRIBONUCLEOSIDE TRIPHOSPHATES

Authors
WU F WANG Y YI M
Citation
F. Wu et al., A SIMPLIFIED METHOD FOR THE PREPARATION OF ALPHA-P-32-LABELED DEOXYRIBONUCLEOSIDE TRIPHOSPHATES, Journal of radioanalytical and nuclear chemistry, 218(1), 1997, pp. 93-95
Citations number
7
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Analytical","Nuclear Sciences & Tecnology
Journal title
Journal of radioanalytical and nuclear chemistry → ACNP
ISSN journal
02365731
Volume
218
Issue
1
Year of publication
1997
Pages
93 - 95
Database
ISI
SICI code
0236-5731(1997)218:1<93:ASMFTP>2.0.ZU;2-M
Abstract
A method for preparing alpha-P-32-labeled deoxyribonucleoside triphosp hates ([alpha-P-32]dNTP) is described. By comparing the labeling proc edure to that of WALSETH et al.(1,2) the reaction of Nuclease P-1 is s aved in our experiments and only three steps are needed for a routine preparation, without decreasing the yield of [alpha-P-32]dNTP which is over 92% based on (32)Pi. In this paper [alpha(32)P]dATP is taken as an example to describe the labeling procedure, the suitability of our method has been discussed.