PARAMETERS CONCERNING THE PREPARATION AND PERFORMANCE OF A MAGNETIC MICROPARTICLE ANTIBODY

We have described ''Magnetic Microparticle Antibodies and Their Applic ation to RIAs'' in a recently published paper.(1) In this article oper ative parameters for the preparation of a magnetic second antibody (MS A-II) including results of purification of donkey anti-rabbit (DxR) se rum by an (NH4)(2)SO4 precipitation method, rates of recovery of produ cts in preparation of magnetic nucleus (MN, Fe3O4 microparticle), in d istillation of acrolein (AL) and in preparation of polyacrolein magnet ic particle (PAMP), change in pH value of suspension irradiated before and after Co-60 gamma-irradiation and volume of wet sediment in separ ation of magnetic particles by a magnetic separator, etc., as well as correlation of levels of quality control (QC) sera obtained with liqui d-phase double antibody assay (LDA) and MSA-II assay during four years were supplementarily summarized. These operative parameters would be helpful to mastering the procedures for preparation and/or use of the magnetic particles. The better correlation of levels of QC sera for bo th the assays showed the reliability of the magnetic antibody.