PREPARATION AND ISOLATION OF A HOMOGENEOUS I-125 CORTISOL DERIVATIVE

Authors
MENON S SAMUEL G BALAKRISHNAN SA
Citation
S. Menon et al., PREPARATION AND ISOLATION OF A HOMOGENEOUS I-125 CORTISOL DERIVATIVE, Journal of radioanalytical and nuclear chemistry, 220(1), 1997, pp. 21-26
Citations number
12
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Analytical","Nuclear Sciences & Tecnology
Journal title
Journal of radioanalytical and nuclear chemistry → ACNP
ISSN journal
02365731
Volume
220
Issue
1
Year of publication
1997
Pages
21 - 26
Database
ISI
SICI code
0236-5731(1997)220:1<21:PAIOAH>2.0.ZU;2-G
Abstract
Tracer specificity plays an important role in the radioimmunoassay (RI A) of steroid hormones. In this paper, we describe the preparation and purification of I-125-labeled cortisol derivative with a carboxymethy loxime-histamine bridge. The investigation on the method of purificati on showed that HPLC could be adopted for the routine preparation of a pure, homogeneous tracer. The retention time observed in HPLC for I-12 5-histamine-CMO-cortisol conjugate could be used as an index for quali tative and quantitative assessments.