PHOSPHINE REDUCED IGG - A NEW METHOD FOR TC-99M LABELING IMMUNOGLOBULINS

A new technetium labeling method for immunoglobulins reduced with tris (2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobul ina IgG source was assayed for purity and optimum reagent's concentrat ion and incubation times were determined. It was purified by column ch romatography and, labelled with Sn2+ reduced technetium in the presenc e of MDP. The kit is easy to prepare, labeling efficiency is > (97 +/- 1.9)% and stable for 6 hours. The immunoreactivity of the Tc-99m-IgG was verified by electrophoresis and Western blot tests. The IgG retain ed its structure after both the reducing and labeling processes and it was the only labeled species.