A METHOD FOR HIGH-FREQUENCY TRANSFORMATION OF TRICHODERMA-VIRIDE

Authors
MANCZINGER L KOMONYI O ANTAL Z FERENCZY L
Citation
L. Manczinger et al., A METHOD FOR HIGH-FREQUENCY TRANSFORMATION OF TRICHODERMA-VIRIDE, Journal of microbiological methods, 29(3), 1997, pp. 207-210
Citations number
14
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
29
Issue
3
Year of publication
1997
Pages
207 - 210
Database
ISI
SICI code
0167-7012(1997)29:3<207:AMFHTO>2.0.ZU;2-E
Abstract
A heterologue plasmid vector-mediated transformation system has been d eveloped for the filamentous fungus Trichoderma viride. Transformants were obtained by using the plasmid pCSN43 carrying the Escherichia col i hygromycin B resistance gene under the control of Aspergillus nidula ns trpC promoter and terminator. The transformation frequency was more than 10(4) mu g(-1) plasmid DNA when nuclease inhibitors were used. H ybridization experiments revealed the presence of replicative plasmids of different sizes in the fungal genome. (C) 1997 Elsevier Science B. V.