SEQUENCE, GENOMIC ORGANIZATION, AND CHROMOSOMAL LOCATION OF THE MOUSEMULLERIAN-INHIBITING SUBSTANCE TYPE-II RECEPTOR GENE

We have determined the sequence and structure of the mouse Mullerian-i nhibiting substance (MIS) type II receptor gene. Sequence comparisons demonstrate that the mouse, rat, rabbit, and human MIS type II recepto rs are highly conserved. The mouse MIS type II receptor gene is encode d by 11 exons and spans approximately 9-kb. Only half of the intron/ex on boundaries of its kinase domain are conserved in comparison to the kinase domain of the related activin type II receptor, Whereas the act ivin type II receptor gene contains large introns (>40-kb), the larges t intron of the MIS type II receptor gene is only 4.3-kb. The MIS type II receptor gene (Amhr) is closely linked to Hoxc on mouse chromosome 15. Knowledge of the sequence and genomic structure of Amhr provides important information for the genetic manipulation of the Amhr locus. (C) 1997 Academic Press.