Ma. Grant, A NEW MEMBRANE FILTRATION MEDIUM FOR SIMULTANEOUS DETECTION AND ENUMERATION OF ESCHERICHIA-COLI AND TOTAL COLIFORMS, Applied and environmental microbiology, 63(9), 1997, pp. 3526-3530
Recovery of total coliforms and Escherichia coli on a new membrane fil
tration (MF) medium was evaluated with 25 water samples from seven sta
tes. Testing of the new medium, m-ColiBlue24 broth, was conducted acco
rding to a U.S. Environmental Protection Agency protocol. For comparis
on, this same protocol was used to measure recovery of total coliforms
and E. coli with two standard MF media, m-Endo broth and mTEC broth.
E. coli recovery on the new medium was also compared to recovery on nu
trient agar supplemented with 4-methylumbelliferyl- beta-D-glucuronide
, Comparison of specificity, sensitivity, false positive error, undete
cted target error, and overall agreement indicated E. coli recovery on
m-ColiBlue24 was superior to recovery on mTEC for all five parameters
. Recovery of total coliforms on the new medium was comparable to reco
very on m-Endo.