SIMULTANEOUS DETERMINATION OF GENE-EXPRESSION AND BACTERIAL IDENTITY IN SINGLE CELLS IN DEFINED MIXTURES OF PURE CULTURES

Authors
POULSEN LK DALTON HM ANGLES ML MARSHALL KC MOLIN S GOODMAN AE
Citation
Lk. Poulsen et al., SIMULTANEOUS DETERMINATION OF GENE-EXPRESSION AND BACTERIAL IDENTITY IN SINGLE CELLS IN DEFINED MIXTURES OF PURE CULTURES, Applied and environmental microbiology, 63(9), 1997, pp. 3698-3702
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
9
Year of publication
1997
Pages
3698 - 3702
Database
ISI
SICI code
0099-2240(1997)63:9<3698:SDOGAB>2.0.ZU;2-S
Abstract
A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in si tu hybridization of Fluorescently labelled oligonucleotide probes to r RNA. The method allows monitoring of gene expression and quantificatio n of beta-galactosidase activity in single cells.