PRODUCTION OF FRUCTOSE-1,6-DIPHOSPHATE (FDP) BY PERMEABILIZED SACCHAROMYCES-CEREVISIAE AND A KINETIC-MODEL FOR FDP ACCUMULATION

Permeable yeast cells were used in the batch production of fructose-l, 6-diphosphate (FDP). The optimum reaction conditions were reported to be: reaction temperature 30 degrees C, toluene concentration 8% (V/V) , and initial ratio of glucose to inorganic phosphorus (Pi) 10:1. Addi tion of AMP was found to be very beneficial to the FDP production. A m ultienzyme system model for FDP accumulation was developed, in which F DP was regarded as a substrate of phosphor-fructokinase (PFK), to simu late the activation effect of FDP on PFK. The model simulations were i n good agreement with the experimental data.