Fluorescence microscopy, X-ray reflectivity and ellipsometry have been
applied to the study of a monolayer of valine-gramicidin A (VGA) at t
he air-water interface to obtain insight into the conformation and ori
entation of VGA, and to clarify the shoulder observed in its pi-A curv
e (12.5-16 mN m(-1), 240-175 Angstrom(2) molecule(-1)). At low molecul
ar areas, the X-ray reflectivities are consistent with formation of a
single layer of tubular-shaped dimers. Fluorescence microscopy yielded
homogeneous pictures suggesting that on the macroscopic scale the sho
ulder cannot be attributed to domain formation. Thicknesses calculated
from the ellipsometric isotherm are consistent with the X-ray data.