DEPOSITION OF ALTERNATING LB MONOLAYERS WITH A NEW TECHNIQUE

Specific features of deposition methods of alternating Langmuir-Blodge tt (LB) monolayers restrict the possibilities of flexible variation of molecule type and conditions of deposition. After pulling out the sub strate from water, molecules of the last monolayer are arranged, as a rule, with inert hydrophobic tails in the direction of the air medium. Thus, for example, the adsorption of another compound onto the active hydrophilic surface of a previously deposited monolayer becomes impos sible. The proposed idea is to protect the sample after dipping down b y a mobile plate situated very near to the surface of the substrate. I f the substrate already closed with the plate is pulled out in air, wa ter is held in the gap by capillary forces and protects the monolayer. Then the closed substrate is transferred to another compartment of th e LB instrument with a solution of the required composition and temper ature for adsorption or deposition. One application of this technique is to include adsorbed layers of soluble proteins into the film withou t denaturation. To check the possibilities of the method, monocomponen t films, alternate-layer structures, and films containing adsorbed pro teins were deposited and studied.