Aa. Kramerov et al., INSECT MUCIN-TYPE GLYCOPROTEIN - IMMUNODETECTION OF THE O-GLYCOSYLATED EPITOPE IN DROSOPHILA-MELANOGASTER CELLS AND TISSUES, Insect biochemistry and molecular biology, 27(6), 1997, pp. 513-521
A mucin-type glycoprotein (GP) from cultured embryonic cells of Drosop
hila melanogaster was isolated and used to raise monoclonal antibodies
(MAbs). Epitope(s) recognized by MAbs were sensitive to the treatment
by O-glycanase, which specifically cleaves off O-linked mucin-type Ga
l(beta 1,3)GalNAc disaccharide, representing the major part of the car
bohydrate moiety of Drosophila GP, Using high-affinity MAbs against ca
rbohydrate epitopes of the Drosophila mucin GP we demonstrated its acc
umulation in culture medium, as well as in cultured cells, which prove
d to be regulated by 20-hydroxyecdysone, Mucin GPs carrying Gal(beta 1
,3)GalNAc disaccharide recognized by the MAbs were immunochemically lo
calized in several Drosophila tissues of ectodermal, mesodermal and ge
rm line origin, including epidermal and follicle cells capable of thei
r secretion, (C) 1997 Elsevier Science Ltd.